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Biomedical Research

Effects of monocular enucleation on the superior colliculus of adult rabbit - A light microscopic study

Author(s): Mohd Asim Khan, Aijaz Ahmed Khan, Nafis Ahmad Faruqi

Vol. 19, No. 1 (1998-01 - 1998-04)

Biomedical Research 2008; 19 (1): 27-31

Mohd Asim Khan, Aijaz Ahmed Khan, Nafis Ahmad Faruqi

Department of Anatomy, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India

Key Words: Superior Colliculus, Monocular Enucleation, Anterograde Degeneration.

Accepted January3, 2008

Abstract

Monocular enucleation induced changes in the visual system in neonates have been reported by many workers but information regarding its effects in adults remains scanty. Present study is an attempt to find out the latter in rabbits. 25 adult rabbits of either sex were subjected to monocular enucleation under general anaesthesia. A similar number of nonenucleates served as controls. Animals were sacrificed after post lesion-survival periods ranging from 2 weeks to 4 months. Brains were fixed by trans-cardiac perfusion of 10% formalin. Superior colliculi were removed from midbrain and processed for paraffin embedding. 10 μ thick sections were stained with Haematoxylin and Eosin, Cresyl violet and Luxol fast blue and Thionin as well as Kluver Barrera and Glees and Marshland stains. To access the deafferentation-induced changes in the contralateral (with respect to enucleation), sections from superior colliculi were observed under light microscope. Morphological changes were in the form of vacuolation in the neuropil, fascicular disorganization and fragmentation of myelin, cytoplasmic and nuclear disintegration of neurons and glial cell aggregation. It was concluded that though the deafferentation-induced changes were not dramatic at any point of time during study, yet its extent could be correlated with duration of post experimental survival period of the animal.

Introduction

Monocular enucleation of rodents soon after birth is known to have profound morphological consequences on the visual centers contralateral to the side of enucleation. This is not surprising as in adult rats and mice about 90% and 97% of the ganglion cell axons cross over at the optic chiasma respectively [1,2]. Morphological studies of the superior colliculus in rodents enucleated soon after birth has been shown to result in deficits in the volume of the colliculus [3,4,5,6,7,8] wherein, the superficial layers being more affected than the deeper one [3,5,9,10]. Enucleation-induced morphological changes during adult hood has also been reported by many workers [3,6,7,11, 12,13,14,15,16] but literature regarding post-lesion time dependent changes in adult superior colliculus remains scanty.

The present study was undertaken to examine the morphological consequences of deafferentation, induced by ocular enucleation in the adult superior colliculus at progressive postlesional intervals.

Materials and Methods

Animals

50 adult healthy rabbits were obtained from the Central Animal House, J. N. Medical College, Aligarh. 25 animals were enucleated for left eye under general anaesthesia by lateral canthotomy procedure and remaining 25 rabbits served as controls. During postoperative period experimental animals were regularly cleaned and dressed for their wounds, and received antibiotics and analgesics. Both experimental (operated) and controls (unoperated) were kept in cages with not more than 3 rabbits per cage. The animals were sacrificed at post operative survival periods of 2 week, 1 month, 2 month and 4 month, and were fixed by intracardiac perfusion of physiological saline followed immediately by 10% formalin solution. Brains were dissected out and the superior colliculi of both sides were removed and were processed for paraffin embedding. 10 μ thick sections were stained with Haematoxylin and Eosin, Cresyl violet, and Luxol fast blue and Thionin as well as Kluver Barrera and Glees and Marshland techniques and observed under light microscope. Relevant findings were recorded in representative photomicrographs.

Results

Gross

Superior colliculi consist of a pair of prominent spheroidal mass of gray matter located on the dorsal aspect of midbrain (Fig.1A). In the experimental animals, up to 2 month of survival, it revealed no obvious size differences. However, at 4 months post enucleation survival, right (contralateral) superior colliculus revealed mild shrinkage both in comparison to left side of the same animal as well as with that of control.

Microscopic Control

It consists of seven laminae (I to VII) from superficial to deep. Each lamina has distinct cytoarchitecture (Fig. 1B). Laminae I and II consist of small and medium sized neurons. Lamina III is composed of medium sized neurons and bundles of nerve fibres (Fig. 2A and 2B) which is said to be of retinocollicular and corticocollicular in origin. Lamina IV consists of medium size neurons with no definite pattern of nerve fascicles. Lamina V is characterized by medium size neurons and definite pattern of nerve fascicles. Only under high power of magnification laminae VI and VII revealed large size multipolar neurons and poorly defined nerve bundle. Glial cells in almost all fields were characterized by small, darkly stained condensed nuclei and absence of Nissl substance in their cytoplasm. They outnumbered neurons in all portions of superior colliculus similar to most regions of brain.

Experimental

Laminae I and II do not reveal obvious change at 2 weeks. At 1 month they show mild vacuolation which was found to increase in size and number with progressive duration of survival and eventually became marked at 4 months (Fig. 3A). It is associated with increasing number of cells that are morphologically comparable to small neurons and glia cells. Deeper laminae also show frequent occurrence of perineuronal spaces. Myelinated fibres which are normally arranged in well defined fascicles, also undergo degenerative changes in the from of fascicular disorganization, fragmentation and ovoid formation (Fig. 3B). Some of the medium sized neurons in samples from 1 month onwards survival revealed disorganization of Nissl substance in the form of detachment of Nissl substance from the neuronal cell membrane (Fig. 3C) in association with aggregation of glia cells, while others showed minimal affects of enucleation (Fig. 3D).

Fig. 1A Fig. 1B

dorsal aspect of thalamus

Fig. 1A: Control - Photograph of dorsal aspect of thalamus (T) and superior colliculus©

coronal section of midbrain

Fig. 1B: Control - Photomicrograph of coronal section of midbrain showing seven cytoarchitectonic laminae (↑↑↑↑). Cresyl violet and Luxol fast blue x25. (For larger image, click here)

Fig. 2A Fig. 2B

superior colliculus

Fig. 2A: Photomicrograph of superior colliculus of control rabbit showing small neurons (↑) in the superficial laminae. Triple stain; XI000

medium sized neuron

Fig. 2B: Photomicrograph from control: showing one medium sized neuron in the centre (٭) and many nerve fibres (thin ↑↑). Triple stain; X1000.

Fig. 3A Fig. 3B

Laminae I and II of superior colliculus

Fig. 3A: Laminae I and II of superior colliculus at 2 months postenucleation showing vacuolations of variable sizes (٭) in the neuropil and small neurons (↑). Haematoxylin-eosin stain; XI000.

Superior colliculus at 4 months

Fig. 3B: Superior colliculus at 4 months postenucleation showing vacuolation (٭), and myelin fragmentation (↑). Triple stain; X1000.

Fig. 3C Fig. 3D

Superior colliculus

Fig. 3C: Superior colliculus of rabbit at one months postenucleation – depicting two medium sized neurons with disorganization of their Nissl substance (٭). Haematoxylineosin stain; X1000.

4 months postenucleation

Fig. 3D: At 4 months postenucleation showing normal looking medium sized neuron in the centre (٭), many small neurons and nerve fibres (↑) from the deeper layer. Silver stain; X1000.

Discussion

Studies dealing with the effects of enucleation in neonates and adult animal [17, 18, 19, 20] have shown changes ranging from an overall shrinkage of the retinorecipient visual center to neuronal shrinkage; neuronal death, decrease neuronal and synaptic density and synapse-to-neuron ratio. Altered neurochemical expressions were also reported by some workers [21]. In the present study observation of mild shrinkage of superior colliculus after chronic deafferentation (4 months) is in agreement with the observation reported in rats [22]. The extent and severity of deafferentation-induced anterograde degeneration was found to be as expected i.e., more pronounced in the contralateral side in accordance with the number of fibres it receives from the retina. In general it was noticed that there was no demonstrable morphological change after 2 weeks of survival while it was very much obvious after 4 months duration. None of the experimental samples revealed cell bearing characteristic features of dead cells. Vacuolations observed in the colliculi of experimental animals are likely to be either due to loss of neuropil and neuronal death or to some extent due to oedema of myelin sheath which are comparable to changes reported in the optic nerve and lateral geniculate nucleus of rabbits [17, 18]. Strikingly conspicuous absence of neuronal cell death debris may be the result of prompt cleaning response of glial cells. Observations on some neurons from deep layers having features analogous to the oedematous neuronal cell body, very akin to postaxotomy perikaryonal response (Fig. 3D), appear unlikely to be an artifact produced by “shattering effects” of histological procedures. Involvement of superficial layers in the present study is very much in agreement with those reported in the adult rats [22]. Our findings on deeper laminae of superior colliculi are correlated well with those reported in adult rat superior colliculus [23]. Relatively mild involvement of the deep layers of the colliculus as compared to superficial ones observed in the present study may be due to the following reasons - (i) superficial layers get direct retinal input hence reveal obvious changes after deafferentation. Deep layers do not receive direct retinal inputs, instead they get afferents from many primary retinorecipient centres e.g., superficial layers of superior colliculus, and visual cortex and hence they show only minimal changes a case of late transsynaptic degeneration. (ii) Loss of synaptic contacts and consequent inactivation of collicular neurons. (iii) Loss of retinal ganglion cell-released trophic factors which is considered essential for the survival of superior collicular neurons. Thus, survival of the neurons of superior colliculus depends on the extent of their dependence on the retinal ganglion cell axons for their activation as well as trophic factors. From above it appears that the superficial layers showing more changes were heavily dependent on the retinal inputs as compared to the deeper layers. It is concluded that though postenucleation changes in different laminae of adult superior colliculus are neither dramatic at any point of time during study nor various morphological changes can be rigidly matched with the postlesion time frame, yet in general it reveals progressive increase in the degenerative changes with duration of post-enucleation survival period in accordance to their extent of dependence on the retinal inputs.

References

  1. Polyak S. The Vertebrate Visual System, Chicago, University of Chicago Press 1957.
  2. Sefton AJ, Dreher B. Visual System, In the Rat Nervous System (ed. Paxinos G), pp. 1169-221 Sydney: Academic Press 1985
  3. Tsang YC. Visual centers in blinded Rats. Journal of Comparative Neurology 1937; 66: 211-261
  4. Delong GR, Sidman RL. Effects of eye removal at birth on his genesis of the mouse superior colliculus: an autoradiographic analysis with tritiated thymidine. J. Comp. Neurol 1962; 118: 205-223.
  5. Terry RJ, Arvor LR, Race EJ. Effect of eye enucleation And eyelid closure upon the brain and associated visual structures in the mouse. IA. Report on degenerative changes. J Exp Zoology 1962; 150: 165-184.
  6. Lund RD, Lund JS. Synaptic adjustment after deafferentation of the superior colliculus of the rat. Science 1971; 171: 804-807.
  7. Lund RD, Lund JS. Modifications of synaptic patterns in the superior colliculus of the rat during development and following differentiation. Vision Research 1971; 11: 281-298.
  8. Lund RD. Cunningham TJ, Lund JS. Modified projections after unilateral eye removal in young rates. Brain behav and Evolution. 1973; 8: 51:72.
  9. Hess A. The experimental Biology of the foetal nervous system. Bio. Rev 1957; 32: 231-260
  10. Hess A. Optic centers and Pathways after eye removal in foetal guinea Pigs. J Comp Neurol 1958; 109: 91-116.
  11. Gless P, Le, Gros. Clark WE. The termination of the optic fibres in the lateral geniculate body of the monkey. J.Anat 1941; 75: 295-310.
  12. Cook WH, Walker JH, Barr ML. A cytological study of transneuronal, Atrophy in the cat and rabbit. J. Comp. Neurol 1951; 94: 267-291.
  13. Golbdy F. A note on transneuronal atrophy in the human lateral Geniculate body. J Neurol Neurosurgery and Psychiatry 1957; 20: 202-207.
  14. Matthews MR, Cowan WM, Powell TPS. Transneuronal cell degeneration in the lateral geniculate nucleus of the macaque monkey . J Anat 1960; 94: 145-169.
  15. Matthews, M. R. Further observations on transneuronal degeneration in the lateral geniculate nucleus of the macaque Monkey. J Anat 1964; 198: 255-363
  16. Kupfer, C. The distribution of cell size in the lateral geniculate nucleus of Man following transneuronal cell atrophy. J. Neuropath. And Exp. Neurol 1965; 24: 653-661.
  17. Khan AA. Wallerian degeneration in the optic nerve of rabbit. Cells Tissues Organs. 2004; 177: 104-109.
  18. Khan AA. Effects of monocular enucleation on the lateral geniculate nucleus (LGN) of rabbit. 2005; Bio-med. Res. 16: 1-5.
  19. Lane RD, Allan DM, Bennett, Clarke CA, Rhoades R. W. Differential age dependent effects of retinal deaferentation upon calbindin and parvalbumin Brain Res. 1996; 740: 208-214
  20. Jeon CJ, Pyun JK, Yang HW. Calretinin and calbindin D28K Immunoreactivity in the superficial layers of the rabbit superior colliculus. Neuroreport 1998; 17: 3847-3852.
  21. Harting JK. Descending pathways form the superior colliculus an autoradiographic analysis in the rhesus monkey (Macaca mulatta). J Comp Neurol 1977; 173: 583-612.
  22. Smith SA, Bedi KS. Unilateral eye enucleation in adult rats causes neuronal loss in the contralateral superior colliculus. J Anat 1977; 190: 481-490.
  23. Labriola AR, laemle LK. Cellular morphology in the visual layer of the developing rat superior colliculus. Exp. Neurol 1977; 55: 247-268.

Correspondence:
Aijaz Ahmed Khan
Department of Anatomy
J. N. Medical College
Aligarh Muslim University
Aligarh 202 002
India

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